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NTS were purified by layering on 4 C Percoll gradient suspension containing Eagles minimum essential medium, penicillin-streptomycin (10,000 U per ml penicillin/10,000 U per ml streptomycin), and 1 M HEPES in 0.85% NaCl with cercariae suspension and centrifuged at 500 g for 15 min

Moreover, immunofluorescence results showed that Cdc42 and PKCz were colocalized on the cell membrane and the colocalization tended to be on one side rather than the uniform distribution throughout the cell membrane after treatment (Fig