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Plates were preloaded with 10 l of laccase dilutions prepared in 20 mM citratephosphate buffer pH 7.0 as follows: 0.28 g ml 1 (LacAnc100) and 0.36 g ml 1 (LaccID) for ABTS, 1.4 g ml 1 (LacAnc100) and 1.8 g ml 1 (LaccID) for DMP, 3.4 g ml 1 (LacAnc100) and 4.0 g ml 1 (LaccID) for guaiacol, 1.7 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid and 340 g ml 1 (LacAnc100) and 405 g ml 1 (LaccID) for p -coumaric acid

ABSTRACT There is significant evidence that the pathogenesis of several neurodegenerative diseases, including Parkinsons disease, Alzheimers disease, Friedreichs ataxia and amyotrophic lateral sclerosis, may involve the generation of reactive oxygen species and mitochondrial dysfunction

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The cardiomyocytes showed characteristic cardiac troponin and actinin structures (Supplementary Figure 1B) and had high purity, as indicated by flow cytometry (Supplementary Figure 1C)