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Middle cerebral artery occlusion Animals in the respective groups underwent transient focal ischemia under isoflurane anesthesia for 60 min by occlusion of the right middle cerebral artery (MCA)

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Prep Time Dose Accuracy Sterility Stability Attach sterile needle to the pen Dial your daily dose Inject subcutaneously near the area of research interest Consistent daily timing optimizes tissue exposure Rotate injection sites to prevent local irritation Store refrigerated between uses Always use a new sterile needle for each administration