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Cells in each treatment group were scratched along the middle of the wells after 4-6 h of transfection, and the dislodged cells were rinsed with PBS, finally, photos were taken at 0 h, 24 h, 48 h, and 72 h

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[1] [2] The pivotal SUSTAIN trials, which evaluated semaglutide for type 2 diabetes, and the STEP trials for weight management did not identify cold intolerance as a statistically significant adverse event