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2, 21 Thick frozen tissue sections (20 m) were incubated for 2 h at room temperature in the incubation solution containing 170 mM Tris-HCl buffer (pH 8.2), 1% bovine serum albumin (BSA), 40 g/ml bacitracin, 10 mM MgCl 2 , and 15 000 c.p.m./100 l 125 I-GLP1(736)amide (2000 Ci/mmol

The analyzed data was blinded, being sent without identification to a third researcher to plot and evaluate the significance

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While research on the relationship between ferroptosis and OA is limited, emerging evidence suggests that ferroptosis plays a crucial role in regulating chondrocyte activity, extracellular matrix degradation, and synovial inflammation, thereby influencing OA progression (Yao et al., 2021)